Protein Kinase B/Akt Induces Resumption of Meiosis in Xenopus Oocytes
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منابع مشابه
Phosphorylation of ARPP19 by protein kinase A prevents meiosis resumption in Xenopus oocytes
During oogenesis, oocytes are arrested in prophase and resume meiosis by activating the kinase Cdk1 upon hormonal stimulation. In all vertebrates, release from prophase arrest relies on protein kinase A (PKA) downregulation and on the dephosphorylation of a long sought but still unidentified substrate. Here we show that ARPP19 is the PKA substrate whose phosphorylation at serine 109 is necessar...
متن کاملBiphasic activation of Aurora-A kinase during the meiosis I- meiosis II transition in Xenopus oocytes.
Xenopus Aurora-A (also known as Eg2) is a member of the Aurora family of mitotic serine/threonine kinases. In Xenopus oocytes, Aurora-A phosphorylates and activates a cytoplasmic mRNA polyadenylation factor (CPEB) and therefore plays a pivotal role in MOS translation. However, hyperphosphorylation and activation of Aurora-A appear to be dependent on maturation-promoting factor (MPF) activation....
متن کاملCalmodulin triggers the resumption of meiosis in amphibian oocytes
The calcium-binding protein, calmodulin, has been purified from Xenopus laevis oocytes. This 18,500-dalton protein, pl 4.3, has two high-affinity calcium-binding sites per mole protein having a dissociation constant of 2.8 x 10(-6) M. Full-grown Xenopus oocytes, arrested in late G2 of the meiotic cell cycle, resumed meiosis when microinjected with 60-80 ng (3-4 pmol) of calmodulin in the form o...
متن کاملThe effect of cdk kinase inhibitors on resumption of meiosis and activation of porcine oocytes
Butyrolactone I (BL I), a potent and specific inhibitor of cyclin-dependent kinases, was shown to block germinal vesicle breakdown (GVBD) in porcine oocytes in a concentration-dependent manner. GVBD was almost totally inhibited over the course of 44 h of culture when 100 pM BL I was included in TCM 199 containing either 3 mg/ml BSA or 5 % fetal calf serum (FCS). In contrast, 50 pM BL 1 was full...
متن کاملEffect of Fibroblastic Growth Factor on Resumption of Meiosis, In Vitro Maturation And Embryo Development of Immature Mouse Oocytes
Purpose: The purpose of this study was to evaluate the effect of fibroblastic growth factor on resumption of meiosis, in vitro maturation of immature mouse oocytes and resulting embryo development with and without basic fibroblastic growth factor-4 (bFGF-4). Materials and Methods: cumulus – oocyte complex (COCs) and germinal vesicle (GV) were obtained from female NMRI mice 46-48 hours after adm...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.30.18705